Anti-active Caspase 3 will label only apoptotic cells, and NOT the 
necrotic cells.  I prefer Caspase to TUNEL for that very reason - TUNEL 
stains everything dying or dead.

Jackie O'




"Patsy Ruegg" 
02/05/2004 02:47 PM

 
        To:     "Bruijntjes, J.P." , 

        cc:     "Ihcrg@Yahoogroups. Com" 
        Subject:        [IHCRG] apoptosis vs necrosis


I am charged with trying to label apoptotic cells in mouse tissue that is
necrotic due to the bacterial infection it has been exposed to.  I have
previously worked extensively with the tunnel assay for apoptosis and 
could
not ever tell the difference between apoptotic cells and necrotic cells. 
Is
there something new that would make this distinctsion?  Please advise

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
Bruijntjes, J.P.
Sent: Thursday, February 05, 2004 1:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)


Hi all

Is anyone of you familiar with a marker for apoptosis called "Cytodeath"
(M30) from Roche on rat tissues?

Joost Bruijntjes
TNO Nutrition and Food Research
PO Box 360
3700 AJ
Zeist
The Netherlands


This e-mail and its contents are subject to the DISCLAIMER at
http://www.tno.nl/disclaimer/email.html

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



 
Yahoo! Groups Links

<*> To visit your group on the web, go to:
     http://groups.yahoo.com/group/IHCRG/

<*> To unsubscribe from this group, send an email to:
     IHCRG-unsubscribe@yahoogroups.com

<*> Your use of Yahoo! Groups is subject to:
     http://docs.yahoo.com/info/terms/
 



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet