We prefer to use a toluidine blue method that does not give any overall
bluish purple background (annoying!) to the other tissue elements, just the
mast cells stain.   Try Churukian Schenk method from J of Histotechnology -
it is a delight, easy to make up and and works on decalcified bone also. 

A toludine blue method for demonstration of mast cells, J Histotechnology,
4:85,1981.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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