[Histonet] IHC background stain
Hello all,
I do all the IHC for our diagnostic veterinary laboratory. Latley i have been getting a lot of background stain from certain protocols. I also over heard one of the pathologists state the sections are getting thicker. Could thick section cause background stain on IHC? I checked my histologist microtome and it is set at 5um. Could her microtome need calibrated?
Roger Charles
Microbiologist
Pennsylvania Veterinary Laboratory
2305 N Cameron St
Harrisburg, PA 17110
717-787-8808
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