-----Original Message-----

We perform IHC on the DAKO Autostainer, at present we am using a
commercial concentrate of TRIS buffer with Tween . We are considering
making our buffers.
I have been told that phosphate buffers are not compatible with alkaline
phosphatase staining systems. Does anyone know of any references to
buffer/alkaline phophatase incompatibility?
Thank you
Rena Fail 
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