[Histonet] Dako ChemMate Envision kit

From:"Anila Syed"

Hi all,

I have been using the Dako Envision ChemMate kit for three weeks with no
success. I wondered whether I was doing something obviously silly like using
the wrong pH. In the protocol it mentions to use the Dako buffer kit. Could
someone tell me whether it suddenly needs to change pH half way through the
I am using PBS at pH 7.4 made up from tablets.

Thanks in advance

Anila Syed

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