methylene blue staining crypts

From:Mike Bromley

methylene blue staining crypts

Hi All

Does anyone have experience staining rat intestine with methylene blue to look for aberrent dysplastic crypts. The mucosal side is examined directly under a dissecting microscope, the method is that of RP Bird from Cancer Letters 37 (1987) 147-151

I am having difficulty differentiating the crypts as everything stains intensely.

Best Wishes

Mike Bromley

Chief Biomedical Scientist
Dumfries & Galloway Royal Infirmary

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