Hello, all. Does anyone
have any suggestions or tips for immunostaining NMDA receptor subunit (and
associated) proteins in 4% parafomaldehyde fixed rat cortex? We currently
use the Vectastain HRP kits (Vector Labs) with DAB as a chromogen. I am
especially interested in hearing about your experiences with different forms
of antigen retrieval (esp. autoclave) or fixation techniques/times that
may improve the signal.
Thanks in advance,
Mary
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Mary B. Olive
Research Assistant
Pediatric Neurology
Children's Hospital of Michigan
Wayne State University