Re: Twort's Gram stain
We routinely use Twort's as outlined in "Carletons Histological Technique"
with the modification that I use acetone to differentiate the crystal
violet and the dehydrating ethanols to differentiate the neutral red in the
Twort's counterstain. We make our own solutions.
At 02:29 PM 02/19/03 -0500, Bartlett, Jeanine wrote:
>Do any of you routinely perform the Twort's Gram procedure? If so, what
>protocol do you use and can the reagents be purchased in kit form?
>Jeanine Bartlett, HT(ASCP)
>Centers for Disease Control
>Infectious Disease Pathology Activity
Mr.Laurie Reilly Ph 07 4781 4468
Physiology & Pharmacology Fax 07 4779 1526
Aust.Inst.of Tropical Vet.& Animal Sc.
James Cook University
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