Re: Twort's Gram stain

From:Laurie Reilly

We routinely use Twort's as outlined in "Carletons Histological Technique" 
with the modification that I use acetone to differentiate the crystal 
violet and the dehydrating ethanols to differentiate the neutral red in the 
Twort's counterstain.  We make our own solutions.

           Regards,    Laurie.

At 02:29 PM 02/19/03 -0500, Bartlett, Jeanine wrote:
>Do any of you routinely perform the Twort's Gram procedure?  If so, what
>protocol do you use and can the reagents be purchased in kit form?
>Jeanine Bartlett, HT(ASCP)
>Centers for Disease Control
>Infectious Disease Pathology Activity
>Atlanta, GA

Mr.Laurie Reilly                                              Ph 07 4781 4468
Physiology & Pharmacology                           Fax  07 4779  1526
Aust.Inst.of Tropical Vet.& Animal Sc.
James Cook University
Townsville  Qld. 


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