|Mateo Ziu <firstname.lastname@example.org>
02/25/2003 04:37 PM
Subject: Ki67 for Human Cells in Mouse tissue
I have been srugling these past weeks with my Ki-67 staining.
I have a cells from a Brain Tumor Human cell line which I injected in the brain of mouse. I have Frozen Section of this animals.
I would like to know if anybody has a good protocol for the Ki-67 staining (Biotinylated. Using DAB staining). What antibody, first and second do you use.
Before I used the Rabbit Anti-Human of DAKO. It worked perfectly. Unfortunatly DAKO doesn't produce it anymore (Ah, these For - Profit Companies!!!).
I have tried with my old protocol many other Antibodies of different Companies, but nothing!!
Please if anybody has a protocol that works in Frosen sections for Ki67, I would appreciate the help.
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