Hi Jane,
How about doing the H & E after the immunostain? If your substrate 
is solvent resistant (like DAB) everything should work out fine! I'm 
sure others with more experience than me will also reply.
Greg

Date sent:      	Mon, 24 Feb 2003 13:57:59 -0600
From:           	Jane Hosmer 
Subject:        	H&E before immuno staining
To:             	histonet@pathology.swmed.edu

> I have a researcher who wants me to stain his slides with H&E before he
> stains with immuno procedure.  After the eosin, should I rinse in any 95%
> alcohols or should I let them air dry immediately after the eosin?   Thanks
> for your help.
> 
> Jane Hosmer, HT,HTL
> CPL Service Center
> UAB Birmingham, Al.
> 
> 


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
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