Re: Blue hue

From:Vinnie Della Speranza


my guess is that the pH of the hematoxylin has shifted . optimal pH is around 2.5 I will bet that your solution's pH is considerably higher than this.
you can correct the pH by adding glacial acetic acid and your stain will be good as new. however I don't recommend doing this blindly without the use of a pH meter to monitor the outcome of adding the acid.

try this and let us know

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, SC 29425
Ph: 843-792-6353
fax: 843-792-8974

>>> "Soto, Roxanne"  02/27/03 09:01PM >>>
Hello out there in histoland--

We have a problem with bluish hue all over out sections and the mucin and
cytoplasm are also staining blue.  We are using Hematoxylin 2 from Richard
Allan.  We have tried everything that we can think of and still are at a
loss--any idea?

Roxanne Soto
AP Supervisor

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