Roxanne,

my guess is that the pH of the hematoxylin has shifted . optimal pH is around 2.5 I will bet that your solution's pH is considerably higher than this.
you can correct the pH by adding glacial acetic acid and your stain will be good as new. however I don't recommend doing this blindly without the use of a pH meter to monitor the outcome of adding the acid.

try this and let us know
Vinnie

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, SC 29425
Ph: 843-792-6353
fax: 843-792-8974

>>> "Soto, Roxanne"  02/27/03 09:01PM >>>
Hello out there in histoland--

We have a problem with bluish hue all over out sections and the mucin and
cytoplasm are also staining blue.  We are using Hematoxylin 2 from Richard
Allan.  We have tried everything that we can think of and still are at a
loss--any idea?

Roxanne Soto
AP Supervisor
414-447-2272
rsoto@covhealth.org 



Privileged/Confidential information may be contained in this message.  The
information contained in this message is intended only for the use of the
recipient(s) named above and their co-workers who are working on the same
matter.

The recipient of this information is prohibited from disclosing the
information to any other party unless this disclosure has been authorized in
advance.

If you are not intended recipient of this message or any agent responsible
for delivery of the message to the intended recipient, you are hereby
notified that any disclosure, copying, distribution or action taken in
reliance on the contents of this message is strictly prohibited.  You should
immediately destroy this message and kindly notify the sender by reply
E-Mail.

Please advise immediately if you or your employer does not consent to
Internet E-Mail for messages of this kind.  Opinions, conclusions and other
information in this message that do not relate to the official business of
the firm shall be understood as neither given nor endorsed by it.