RE: very large parffin embedment molds
Surgipath sells super cassettes, dimensions 3in. x 2in. by .75in high. They also sell the corresponding base molds and chuck adaptor. Phone 1-800-225-3035. I don't think they have large slides, but its worth asking. You could, if all else fails, find a thickness of glass that will work,at a hardware store and break your own using a hand scoring instrument. As far as processing, utilize a dehydrating solution that is 70% ablsolute alcohol and 30% xylene. I use this on my processer and get thorough infiltrating on some good size chunks of tissue. I have left tissue on delay in this solution for 10 days without ill effects.
Sent: Monday, February 24, 2003 2:28 PM
Subject: very large parffin embedment molds
I have to embed some sheep organs, whole. Does anyone know of a source for
large embedment molds, slides, coverslips, cassettes, etc.?
Also, what is the stratagy for large organs? Is drying out the edges while
the middle is infiltrating a problem, if so how does one get around it?
Thanks for your help,
Kathy Walters //
Central Microscopy Research Facility / /
85 EMRB / /\
University of Iowa / /\ \
Iowa City, Iowa 52242 / / \ \
Phone #: (319) 335 8142 / / \ \
Fax #: (319) 384 4469 ______ ((0))
email: Katherine Walters@uiowa.edu |__| / /
|| / /
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