RE: mast cell staining

From:"BORTOLOTTO Susan (SVHM)"

Thank you to all the replies regarding the staining of mast cells.  I do regulary use histochemical stains, including Toluidine Blue, Alcian Blue and Chloroacetate Esterase.  These are good for mature mast cells, but I was unsure if I was able to see immature mast cells, as the granules are not yet visible, hence my thought of using c-kit antibody.
   

Susan Bortolotto, PhD
Research Officer
Bernard O'Brien Institute of Microsurgery
42 Fitzroy St.
Fitzroy, 3056
AUSTRALIA




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