RE:Ki67 for Human Cells in Mouse tissue
I have been struggling for the same problem with double staining using a
rabbit anti-Ki67 and mouse antibody. A couple of weeks ago the problem was
solved. Our NeoMarkers (Lab Vision Corp.) rep came in with a new rabbit
monoclonal (you read this right) anti-Ki67. Clone SP6, product number
RM-9106-R2. I tested the stuff and found out this antibody works fine
indeed, maybe even better as the old Dako rabbit anti-Ki56!
Chris van der Loos
Academic Medical Center
Dept. of Cardiovascular Pathology
Amsterdam, The Netherlands
Mateo Ziu wrote:
>Date: 25 Feb 2003 17:00:35 -0600
>From: Mateo Ziu
>Subject: Ki67 for Human Cells in Mouse tissue
>I have been srugling these past weeks with my Ki-67 staining.
>I have a cells from a Brain Tumor Human cell line which I injected in the
>brain of mouse. I have Frozen Section of this animals.
>I would like to know if anybody has a good protocol for the Ki-67 staining
>(Biotinylated. Using DAB staining). What antibody, first and second do you
>Before I used the Rabbit Anti-Human of DAKO. It worked perfectly.
>DAKO doesn't produce it anymore (Ah, these For - Profit Companies!!!).
>I have tried with my old protocol many other Antibodies of different
>Companies, but nothing!!
>Please if anybody has a protocol that works in Frosen sections for Ki67, I
>would appreciate the help.
>Brigham and Women's Hospital
>LMRC Bldg, Rm 121
>Boston, MA 02115 (USA)
<< Previous Message | Next Message >>