RE: Criteria for staining Cytology specimens with high potential of c ross-contamination
I've always been told by the Cytotechs that I work with that you can use the
same Non-Gyn staining line for all specimens, just stain the specimens that
are suspicous last and then filter all solutions. This has satisfied CAP
all of my years processing Non-Gyn specmiens.
So, if I would have two urines and then a body fluid highly suspicous you
could stain the urines together and then stain the body fluid. You would
then filter every staining dish in the line.
Hope this helps.
From: Landrum, Donna (San Jac) [mailto:DLandrum@tmh.tmc.edu]
Sent: Monday, February 17, 2003 10:47 AM
Subject: Criteria for staining Cytology specimens with high potential of c
The lab I work in is small. We process surgical specimens and non-gyn
cytology. I recently have had to create a new procedure on how we batch and
stain our cytology. We have to separate low-cellular, high cellular, and
FNA's. They have to all be stained separately and in their own set-up. We
do PAP's and H&E's on most all cytologies.
My question is, Does anyone have any tips on how to keep this simplified?
Currently, I am staining all PAP's together and all H&E's together. If I
have to separate, I would have to set up six different staining set-ups. I
do not have an automatic stainer right now, so these are all manual set-ups.
My feeling is that this is total overkill. Especially since we have never
had any problems. But, to satisfy CAP, we must separate everything.
If you have any helpful information, I would appreciate any ideas.
Donna Landrum, HT (ASCP)
Histology Lab Supervisor
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