RE: Bielchowsky stain

From:Andrew Shand

Bielchowsky stain
We do Modified Bielschowsky which as you suggest is an expensive method, so I'd be very interested to have details of your cheap protocol.
Bob Quilty
Dept of Neuropathology
Frenchay Hospital, Bristol, UK.
-----Original Message-----
From: Featherstone, Annette [mailto:AFeatherstone@KaleidaHealth.Org]
Sent: 14 February 2003 17:04
To: 'Nymeyer, Heather'; ''
Subject: RE: Bielchowsky stain

We use a modified Bielschowsky stain that uses much less silver nitrate. The stain is consistent and much, much cheaper. The whole stain only uses 5.9ml of "1%" silver nitrate. The stain is watched for development and taken out before it becomes over stained. Let me know if you would like this protocol
Annette Featherstone HT/ Kalieda Health, Buffalo NY

-----Original Message-----
From: Nymeyer, Heather []
Sent: Thursday, February 13, 2003 19:49
To: ''
Subject: Bielchowsky stain

I need some help in reducing or eliminating the amount of background silver precipitate on the slide when performing a Bielchowsky stain. The method being used is a 20 % silver nitrate solution for 20 mins followed by a 15 min incubation (in the dark) of ammoniacal silver.  The reducer consisting of citric acid, formaldehyde, water.  A few drops of the reducer is added to the ammoniacal silver and checked microscopically until fibers are seen.  At the same time the fibers are being developed the excess background precipitate is forming.    Any suggestions or solutions would be greatly appreciated. 

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