Mike

I've used the same protocol (R.P. Bird) quite often and with good
results. In the beginning the staining was too strong so I reduced the
concentration to 0.1% dissolved in PBS or even methanol. And the maximum
time of staining was at best about 7 minutes. After dissolving the
methylene blue I filtered this solution before I used it.
I hope this will help you.

Joost Bruyntjes
TNO Nutrition and Food
Zeist


-----Original Message-----
From: Mike Bromley [mailto:M.Bromley@dgri.scot.nhs.uk] 
Sent: woensdag 26 februari 2003 16:22
To: 'histonet@pathology.swmed.edu'
Subject: 

Hi All 
Does anyone have experience staining rat intestine with methylene blue
to look for aberrent dysplastic crypts. The mucosal side is examined
directly under a dissecting microscope, the method is that of RP Bird
from Cancer Letters 37 (1987) 147-151
I am having difficulty differentiating the crypts as everything stains
intensely 

Best Wishes 
Mike Bromley 
Chief Biomedical Scientist 
Pathology 
Dumfries & Galloway Royal Infirmary 
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