From:"Bruyntjes, J.P. "


I've used the same protocol (R.P. Bird) quite often and with good
results. In the beginning the staining was too strong so I reduced the
concentration to 0.1% dissolved in PBS or even methanol. And the maximum
time of staining was at best about 7 minutes. After dissolving the
methylene blue I filtered this solution before I used it.
I hope this will help you.

Joost Bruyntjes
TNO Nutrition and Food

-----Original Message-----
From: Mike Bromley [] 
Sent: woensdag 26 februari 2003 16:22
To: ''

Hi All 
Does anyone have experience staining rat intestine with methylene blue
to look for aberrent dysplastic crypts. The mucosal side is examined
directly under a dissecting microscope, the method is that of RP Bird
from Cancer Letters 37 (1987) 147-151
I am having difficulty differentiating the crypts as everything stains

Best Wishes 
Mike Bromley 
Chief Biomedical Scientist 
Dumfries & Galloway Royal Infirmary 
This e-mail and any files transmitted with it are private and intended 
solely for the use of the individual or entity to whom they are
If you have received this e-mail in error please return it to the
address it 
came from telling them it is not for you and then delete it from your 

<< Previous Message | Next Message >>