Preadipocyte factor-1 expression in muscle fibres
I have developed an immunohistochemical staining procedure to locate
preadipocytes in bovine muscle tissue by immunolocating preadipocyte
factor-1 (pref-1). Pref-1 is a transmembrane protein known to be
expressed in preadipocytes but not in fully differentiated adipocytes.
During my staining procedure development, I often viewed pref-1 to be
'expressed' in individual muscle fibres. Clearly the expression was
seperate from the connective tissue (perimycium) where adipocytes are
known to develop. No clear explanation, however, was ever developed for
why pref-1 would be expressed in muscle fibres. I have posted a few
images of the sections (on histonet.org) that I obtained and I would
enjoy comments on perhaps why I am seeing this expression. If people
would like to know the protocol please let me know. I am basically
curious if I am seeing artifacts of the fluorescence staining or if
anyone has obtained similar fluoresence staining when working with
muscle sections.
Thanks
Phil
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