Hello everybody,

I have been srugling these past weeks with my Ki-67 staining.

I have a cells from a Brain Tumor Human cell line which I injected in the brain of mouse. I have Frozen Section of this animals.

I would like to know if anybody has a good protocol for the Ki-67 staining (Biotinylated. Using DAB staining). What antibody, first and second do you use.

Before I used the Rabbit Anti-Human of DAKO. It worked perfectly. Unfortunatly DAKO doesn't produce it anymore (Ah, these For - Profit Companies!!!).

I have tried with my old protocol many other Antibodies of different Companies, but nothing!!

Please if anybody has a protocol that works in Frosen sections for Ki67, I would appreciate the help.

Mateo Ziu

Neurosurgery Lab

Brigham and Women's Hospital

LMRC Bldg, Rm 121

Boston, MA 02115 (USA)

Tel. (617) - 278 0176

Fax. (617) - 232 9029