GFAP and NeuN on mouse tissue?

From:Melissa Gonzalez

Does anyone have any experience using Chemicon's mouse anti-NeuN and/or DAKO's rabbit anti cow GFAP?
I have seen a few discussions relating to FFPE human tissue, but we are working on mouse frozens for fluorescence microscopy..which leads into my next question, How thick can I keep (or how thin should I cut) the tissue (brain) for optimal visualization?

Thanks in advance for your help!

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