Cloudiness
Incomplete dehydration or water contamination of
your last absolute alcohol or xylene will result in cloudiness as will use of
too much mounting medium. Take a slide wet with clean xylene and place three
drops of resinous mounting medium over the tissue where they will spread in the
xylene. Place the coverslip on an angle one edge first and gently
lower and blot excess fluid by placing the edge on a gauze or paper towel. Press
bubbles gently out with needle. No fingerprints.
Jeff Silverman
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