Brown Fat in Plastics

From:Judi Ford

Hi to everyone out there!

I have an investigator who may be interested in processing brown fat for plastic sectioning.  We use Technovit as our resin. Is this reasonable to do (I'm relatively new to the whole world of plastcs)? If so, are there problems with dehydrating the tissue through the alcohol (like destroying the fat), before infiltrating with resin.  Is there a substitute dehydrant that will work with Technovit?

The other question he wants to know, assuming that you can process brown fat for plastic sectioning, is if you can do an Oil Red O on the tissue?  If not, is there a stain for fat plastic sections?

Any help sent this way is greatly appreciated. 


Judi Ford
Jackson Laboratory
Bar Harbor, ME

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