Hi. After my immunostaining procedure that ends with three changes in xylene, I coverslip (using Cytoseal, Richard Allen Scientific). When I look at the slides under the microscope I notice some "cloudiness." I'm wondering if that is due to the xylene drying off the tissue or due to the xylene still being on the tissue. Also, I'm not sure if it's the cytoseal. I'm using it for the first time, so if anyone had problems with it, please let me know. Thank you for your time.

Nidal E Muvarak
Associate Research Specialist
Department of Biomedical Engineering
University of Wisconsin-Madison
1550 Engineering Dr.; Rm. 2158
Madison, WI 53706-1609
Lab: (608) 265-8921; Office: (608) 265-4205; 
Home: (608) 256-7934; Cell: (608) 332-6068

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