quenching neuronal autofluorescent

From:Carol Bobrowitz

We are using paraformaldehyde to perfuse goat brainstem for indirect
fluorescent (FITC) immunohistochemical analysis.
The frozen sections (25um) are mounted on chrome-gelatin treated slides.  
After staining a slide thru the immuno procedure with absolutely no antibody
(primary or secondary) application, we found high levels of neuron-specific
Is there some chemical we can apply or treatment we can perform to quench
the autofluorescence?
Any help or suggestions will be greatly appreciated.

Carol Ann Bobrowitz

Department of Physiology
Medical College of Wisconsin
Milwaukee, Wisconsin
(414) 456-8179
FAX (414) 456-6546

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