brain morphology stain

From:Matt McElwee

Hello Histonetters.
I'm looking for a good morphology stain to use on free floating (35
micron) mouse brain sections.  I'm particularly interested in a stain
that clearly shows the amygdala and the midbrain.  The only catch is
that it can't autofluoresce.   Does any one know of a good stain for
this?  I would be willing to use either a stain that's not the greatest
but doesn't autofluoresce or does autofluoresces but is great for
viewing the amygdala and midbrain.  Any help would be greatly
appreciated.  Thank you.

Matt McElwee
Research Specialist
Department of Surgery
K4/617 Clinical Science Center
600 Highland Avenue
Madison, WI 53792
(608) 263-7648

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