auto - fluorescence
Does anyone can answer this question?
Normally we do the IHC Free Floating Method on vibratome sections. No
problems. Then we did a pilot with paraffin sections. All the sections gave
an auto -fluorescence. Even without doing the IHC itself, just
deparrafinize, hydrating to water gives already this auto-fluorescence.
How does this occur?
Dept. Cellular Animal Physiology
University of Nijmegen
6525 ED Nijmegen
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