Stain fro streptococcal cell wall

From:"Kinsley, David"

Stain fro streptococcal cell wall

Hi all,

I need to do a stain for streptococcal bacteria cell wall in an inflamed joint and would like to know which staining method is the most optimal, easiest, safest to perform.  I have been looking at the Gram stain, Brown and Brenn as well as the Brown and Hopps staining methods, but fear I may have a safety issue with ordering picric acid for my lab. 

Any suggestions?  Are there any effective substitutions for picric acid steps?

Thanks in advance,

Dave Kinsley
Schering Plough Research Institute
Kenilworth, NJ 07033


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