Re: type of paraffin

From:Nancy E Weber

Hi Debbie,
I used to cut rat testis at 10 and sometimes 20 microns for a researcher and
found that if I put the blocks on the ice tray they would always curl up and
I could not get a good section.  But I found that if I let the blocks get to
room temp or close to it , they would lay right out off the knife and there
was no distortion to the section.  Might give it a try and see if that

Nancy Weber
Research Associate
Veterinary Clinical Sciences
Ohio State University
  ----- Original Message -----
  From: Grant, Debra
  To: Histonet (E-mail)
  Sent: Thursday, February 06, 2003 11:37 AM
  Subject: type of paraffin

  Hello Histonetters,

  We are having trouble cutting thick paraffin sections of mouse embryo, the
sections will not ribbon and they curl up.
  Can anyone suggest a good paraffin to use? We are using Shandon, Precision
cut paraffin. Thanks in advance!

  Debby Grant
  Research Technician II
  Histology Core Facility
  Stowers Institute for Medical Research
  1000 E. 50th Street
  Kansas City, MO 64110

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