Re: quenching neuronal autofluorescent
The only autofluorescence I've seen in brain tissue is/are erythrocytes.
You can tell that by morphology. What does what you're looking at look
John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL 36849
On Wed, 12 Feb 2003, Carol Bobrowitz wrote:
> We are using paraformaldehyde to perfuse goat brainstem for indirect
> fluorescent (FITC) immunohistochemical analysis.
> The frozen sections (25um) are mounted on chrome-gelatin treated slides.
> After staining a slide thru the immuno procedure with absolutely no antibody
> (primary or secondary) application, we found high levels of neuron-specific
> Is there some chemical we can apply or treatment we can perform to quench
> the autofluorescence?
> Any help or suggestions will be greatly appreciated.
> Carol Ann Bobrowitz
> Department of Physiology
> Medical College of Wisconsin
> Milwaukee, Wisconsin
> (414) 456-8179
> FAX (414) 456-6546
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