Use the widest needle you can find, at least
1mm diameter. File off the tip to make it
blunt, then you won't poke it through the
other side of the vessel.
-- 
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan@uwo.ca
   http://publish.uwo.ca/~jkiernan/
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Cristiano Rumio wrote:
> 
> Dear Friends,
> 
> some of you have an idea of the diameter of a needle to perfuse a adult rat
> via aorta?
> 
> Thank you in advance
> 
> Cristiano Rumio
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