Re: favorite mounting media for IHC

From:Geoff McAuliffe

DPX. Also, DAB that has not been intensified with gold, silver or osmium tends
to fade, at least in my experience.


Carrie Kyle-Byrne wrote:

> hi all,
> we're noticing some significant fading of chromagen and hematoxylin on our
> IHC are some particulars......we use xylene, our DAB is from
> Dako, hematoxylin is Mayer's from Sigma, and we currently use CytoSeal.  i'm
> looking to switch to a different media......what's your favorite for good
> long term storage.
> thanks for your input,
> carrie
> ps.....thank goodness we take pictures of everything for the much loathed
> lab notebook!

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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