Re: beta-hydroxysteroid dehydrogenase

From:"J. A. Kiernan"

See Van Noorden CJF & Frederiks WM 2002. Metabolic mapping
by enzyme histochemistry. Chapter 11, pp 269-278 in
"Microscopy and Histology for Molecular Biologists: A User's
London: Portland Press (ISBN 1855781417).
It won't work on paraffin sections. For dehydrogenase
histochemistry you need unfixed cryosections. Steroid
dehydrogenases will withstand brief treatment of the
sections with acetone to remove lipids, according to
Lojda, Gossrau & Schiebler's "Enzyme Histochemistry"
book (1979), p.270-272.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
"Wilhelms, Margaret B" wrote:
> Does anyone do beta-hydroxysteroid dehydrogenase enzyme histochemistry on
> formalin fixed paraffin embedded tissues?  I would appreciate any help with
> this.  Thanks in advance.
> Meg

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