I use the anti-human RSV monoclonal from NovoCastra (NCL-RSV3) at 1:300 with
microwaving in 0.01M citrate buffer for 20 minutes (4 x 5 minutes).  It
works beautifully... but you need a good + control.  Be sure to put 2%
normal bovine serum in your linking Ab to block binding to the endogenous
Igs in the lung.

Jan Shivers
Univ of Minn Vet Diag Lab

----- Original Message -----
From: "Greg Dobbin" 
To: 
Cc: 
Sent: Tuesday, February 11, 2003 11:31 AM
Subject: Veterinary Viral Ab's


> Hello All,
> I'm wondering if any of you fellow Vet Medicine types know of a
> source of antibodies (Poly- or Monoclonal) to Porcine Cirocovirus
> and Bovine Respiratoty Syncytial Virus (BRSV)?
>
> I see our friends at VMRD have Direct FA conjugates to these;
> which leads me to my next question: what is to be expected from
> staining formalin-fixed paraffin embedded tissue sections with
> direct FA ab's? (I think I'll re-post this particular question to the list
> with the subject: "Direct FA on FFPE", to get a wider response. My
> apologies in advance to those of you who end up opening both
> e-mails!)
>
> I seem to remember trying this at one time (when I worked in a
> virology lab) and had good results. Does it really depend on the
> antigen in question and how it responds to fixation and processing?
>
> Any and all responses welcome!
> Cheers!
> Greg
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> Greg Dobbin
> Pathology Lab
> Atlantic Veterinary College, U.P.E.I.
> 550 University Ave.
> Charlottetown, P.E.I.
> Canada,  C1A 4P3
> Phone: (902)566-0744
> Fax: (902)566-0851
> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
> "A farmer is a person outstanding in their field."
>
>