Re: Luxol Fast Blue (LFB) and OCT

From:"J. A. Kiernan"


Either you or your boss need to read up on the basics
of myelin staining. There are 3 different luxol 
fast blue dyes: ARN, G and MBS. Their similarities 
are their blueness and their being salts of coloured 
hydrophobic anions with hydrophobic cations. Such
salts dissolve in alcohol but not in water. The
technology of using these dyes to stain myelin,
which is a hydrophilic lipid, depends critically 
on both the actual dye used and the mixture of 
solvents from which it is applied. 

It is possible to get spectacular myelin staining
with "luxol" dyes, but why use compounds of
uncertain identity, some of which haven't been
manufactured for many years?  Myelin can be
stained easily with inexpensive non-secret dyes 
that have been available for 100 years. It's 
all in the textbooks: recent ones and ones that
pre-date the introduction of a "luxol" blue
by Kluver & Barrera in 1953.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1

David Chen wrote:
> Hi, I was wondering if somebody can give me a protocol for
> staining the myelin that has been frozen in OCT with luxol fast
> blue? Thank you for your help. It is very much appreciated.
> David Chen
> University of California Irvine
> ---------------------------------------------------------------
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