Re: IHC for Acetylcholinesterase?
AChE activity is demonstrated by enzyme activity
histochemistry in frozen sections, not by
immunohistochemistry. There are several methods.
Pearse & Stoward's "Histochemistry" Vol 3 has
a substantial chapter on the subject, with
instructions for several techniques.
The neurons in enteric ganglia also show nicely with
trichrome techniques applied to paraffin sections.
More can be seen in longitudinal than in transverse
sections. With either method you'll need a bit of
positive control gut, (a) to be sure that the method
is working properly, and (b) to get your eye in
practice for spotting the ganglia.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
Greg Dobbin wrote:
> A pathologist at my facility would like to know if anyone is doing
> immunohistochemistry (IHC) to detect acetylcholinesterase activity
> as a diagnostic indicator for agangliosis (i.e. absence of activity
> supports the diagnosis) or for any other reason for that matter (e.g
> We are interested in hearing from any labs doing such staining
> (i.e. any and all species); but I will add that her patient is a
> post-mortem cow.
<< Previous Message | Next Message >>