I used surgipaths embedding medium at my old lab and
had allot of trouble with particles and discoloration,
at my new facility they use Richard-Allans embedding
medium in the processor for infilitration and for
embedding tissue and it works well.  I would give that
a try too.

--- HistoNet Server 
wrote:
> 
>
----------------------------------------------------------------------
> 
> Date: 7 Feb 2003 05:15:43 -0600
> From: FANDORFP@aol.com
> Subject: Re: WAGE SURVEY
> 
> 
> The ASCP has a salary survey from 2001. Go to their
> website.
> 
> 
> 
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
> 
> 
> - ------------------ MIME Information follows
> ------------------
> 
> 
> - --Boundary_(ID_e1mf5HboFhvVA+xUdi+5FQ)
> Content-type: text/plain; charset=US-ASCII
> Content-transfer-encoding: 7BIT
> 
> <<<<<< See above "Message Body" >>>>>>
> 
> - --Boundary_(ID_e1mf5HboFhvVA+xUdi+5FQ)
> Content-type: text/html; charset=US-ASCII
> Content-transfer-encoding: 7BIT
> 
>  FAMILY="SANSSERIF" FACE="Arial"
> LANG="0">The ASCP has a salary survey from 2001. Go
> to their
> website.
> 
> - --Boundary_(ID_e1mf5HboFhvVA+xUdi+5FQ)--
> 
> 
>
----------------------------------------------------------------------
> 
> Date: 7 Feb 2003 07:15:53 -0600
> From: JaynesF@pediatrics.ohio-state.edu
> Subject: RE: type of paraffin
> 
> I had terrible times trying to cut mouse tissue,
> finally I learned to soak
> them in Ice water (Mostly ice,but yet liquidy) for a
> long time 30 min to 1
> hour . I get great sections even  on mouse eyes.
> 
> Best of luck
> 
> F. Jaynes
> Biopathology Laboratory
> Children's Hospital Reaearch
> Columbus, ohio
> 
> 
> 
> 
> 
> 
> 
> 
> 
> >   Hello Histonetters,
> > 
> >   We are having trouble cutting thick paraffin
> sections of mouse embryo,
> > the
> > sections will not ribbon and they curl up.
> >   Can anyone suggest a good paraffin to use? We
> are using Shandon,
> > Precision
> > cut paraffin. Thanks in advance!
> > 
> >   Debby Grant
> >   Research Technician II
> >   Histology Core Facility
> >   Stowers Institute for Medical Research
> >   1000 E. 50th Street
> >   Kansas City, MO 64110
> >   drg@stowers-institute.org
> > 
> > 
> > 
> 
> 
>
----------------------------------------------------------------------
> 
> Date: 7 Feb 2003 08:15:47 -0600
> From: "Wilhelms, Margaret B"
> 
> Subject: beta-hydroxysteroid dehydrogenase
> 
> Does anyone do beta-hydroxysteroid dehydrogenase
> enzyme histochemistry on
> formalin fixed paraffin embedded tissues?  I would
> appreciate any help with
> this.  Thanks in advance.
> 
> Meg
> 
> 
> 
> LEGAL NOTICE
> Unless expressly stated otherwise, this message is
> confidential and may be
> privileged. It is intended for the addressee(s)
> only. Access to this E-mail by
> anyone else is unauthorized. If you are not an
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> copying of the contents of this E-mail or any action
> taken (or not taken) in
> reliance on it is unauthorized and may be unlawful.
> If you are not an
> addressee, please inform the sender immediately.
> 
> 
>
----------------------------------------------------------------------
> 
> Date: 7 Feb 2003 08:45:10 -0600
> From: Mary_Georger@URMC.Rochester.edu
> Subject: RE: GAP 43
> 
> Elizabeth,
> I do Connexin 43, which is a gap junction protein
> antibody, on FFPE tissue.
> If this is what you are looking for give me a shout
> and I will forward the
> protocol on to you.
> 
> Mary Georger
> Center for Cardiovascular Research
> University of Rochester Medical Center
> KMRB Room 2-9816
> 601 Elmwood Avenue.
> PO Box 679
> Rochester, New York  14642
> 585-273-1548
> 
> > ----------
> > From: 	Elizabeth Chlipala
> > Sent: 	Thursday, February 6, 2003 11:59 AM
> > To: 	HistoNet Server
> > Subject: 	GAP 43
> > 
> > Has anyone out there done immunostaining for GAP
> 43 on 40 micron vibratome
> > brain sections?  Also I have never worked with
> vibratome sections or done
> > work on free floating sections.  Is there a
> reference or protocol with a
> > list of supplies, so I can see what I'm getting
> into and what I would need
> > to purchase.
> > 
> > Thanks in advance
> > 
> > Liz Chlipala
> > BolderPATH
> > 
> > 
> > 
> 
> 
>
----------------------------------------------------------------------
> 
> Date: 7 Feb 2003 09:30:18 -0600
> From: nyilmaz@mersin.edu.tr
> Subject: daily digest
> 
> daily digest
> 
> 
>
----------------------------------------------------------------------
> 
> Date: 7 Feb 2003 09:30:42 -0600
> From: Fred Underwood 
> Subject: RE: cassette labeling system
> 
> I have used the Surgipath VCP printer.  It can be
> programmed to print numbers
> in sequence or repeat the number.  Screens for
> different accession numbers can
> also be programmed .
>  
> Fred Underwood
> 
> 	-----Original Message-----
> From:  
> Sent: Thursday, February 06, 2003 8:48 PM
> To: 
> Subject: cassette labeling system
> 
> 
> 
> 
=== message truncated ===


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