Re: Borax treatment & Grad Supervision

From:"J. A. Kiernan"

In answer to the oblique/anonymous question:

What the graduate student should be asking is: 

NOT  "Does anyone have a Borax treatment protocol ..."

BUT  "Will someone please explain what borax is and what it does
to DNA, because my supervisor does not know and he cannot be
bothered to go to the library and find out."

Has a supervisor advised a student to do experiments based on
answers to a question sent out on the Internet?  If so:

The supervisor deserves dismissal from his university for not
doing his job. That is unlikely to happen.

The student needs to find a new supervisor who knows the field
and is willing and able to do the supervising.

John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
David Walker wrote:
> I am posting this for a Graduate Student:
> Dear Histonetters-
> I am doing a double-labeling immunohistochemistry protocol for smooth muscle
> and BrdU.  The method calls for an HCl treatment to denature the DNA
> followed by a Borax treatment to stabilize the DNA.  Does anyone have a
> Borax treatment protocol, including recipes?  Thank you in advance for your
> responses.
> Regards,
> Taren Grass
> Dave

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