RE: type of paraffin

From:Tony Henwood

RE: type of paraffin


This seems like a classic case of over-processing.

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: Jaynes, Florinda []
Sent: Saturday, 8 February 2003 0:02
To: 'Nancy E Weber'; Grant, Debra
Cc: histonet
Subject: RE: type of paraffin

I had terrible times trying to cut mouse tissue, finally I learned to soak
them in Ice water (Mostly ice,but yet liquidy) for a long time 30 min to 1
hour . I get great sections even  on mouse eyes.

Best of luck

F. Jaynes
Biopathology Laboratory
Children's Hospital Reaearch
Columbus, ohio

>   Hello Histonetters,
>   We are having trouble cutting thick paraffin sections of mouse embryo,
> the
> sections will not ribbon and they curl up.
>   Can anyone suggest a good paraffin to use? We are using Shandon,
> Precision
> cut paraffin. Thanks in advance!
>   Debby Grant
>   Research Technician II
>   Histology Core Facility
>   Stowers Institute for Medical Research
>   1000 E. 50th Street
>   Kansas City, MO 64110

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