RE: type of paraffin
I had terrible times trying to cut mouse tissue, finally I learned to soak
them in Ice water (Mostly ice,but yet liquidy) for a long time 30 min to 1
hour . I get great sections even on mouse eyes.
Best of luck
F. Jaynes
Biopathology Laboratory
Children's Hospital Reaearch
Columbus, ohio
> Hello Histonetters,
>
> We are having trouble cutting thick paraffin sections of mouse embryo,
> the
> sections will not ribbon and they curl up.
> Can anyone suggest a good paraffin to use? We are using Shandon,
> Precision
> cut paraffin. Thanks in advance!
>
> Debby Grant
> Research Technician II
> Histology Core Facility
> Stowers Institute for Medical Research
> 1000 E. 50th Street
> Kansas City, MO 64110
> drg@stowers-institute.org
>
>
>
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