RE: quenching neuronal autofluorescent

From:"Charles W. Scouten, Ph.D."

Perfusion with high concentrations of paraformaldehyde/glutaraldehyde
will make catecholamines fluoresce in the rat brain, but this it not
seen using the 4% solution commonly mixed.  Are you using a high
concentration, or usual mix?


Charles W.  Scouten, Ph.D.
5918 Evergreen Blvd.
St. Louis, MO 63134
Ph: 314 522 0300  
FAX  314 522 0377

-----Original Message-----
From: Carol Bobrowitz [] 
Sent: Wednesday, February 12, 2003 2:41 PM
To: HISTONET (E-mail)
Subject: quenching neuronal autofluorescent

We are using paraformaldehyde to perfuse goat brainstem for indirect
fluorescent (FITC) immunohistochemical analysis.
The frozen sections (25um) are mounted on chrome-gelatin treated slides.

After staining a slide thru the immuno procedure with absolutely no
(primary or secondary) application, we found high levels of
Is there some chemical we can apply or treatment we can perform to
the autofluorescence?
Any help or suggestions will be greatly appreciated.

Carol Ann Bobrowitz

Department of Physiology
Medical College of Wisconsin
Milwaukee, Wisconsin
(414) 456-8179
FAX (414) 456-6546

<< Previous Message | Next Message >>