RE: attaching MMA thin sections to slides
What is the benefit of floating out sections on a slide versus rolling them with plastic and clamping them (besides being quicker)? I tried floating out PMMA sections and found they wrinkled much more than flattening them with a roller. I didn't try the technique of using a hot plate. In the technique below, the slides do not lay flat on the hot plate but are propped up so the water drains out from beneath the section? And, is it ok to dry cut PMMA blocks?
Teri Johnson (The PMMA novice)
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri 64110
From: Karen Pawlowski [mailto:firstname.lastname@example.org]
Sent: Wednesday, February 12, 2003 9:00 AM
To: Sarah Jones
Subject: Re: attaching MMA thin sections to slides
We have good luck with 0.5 to 6 micron thin sections using the following
1) section the 4-6 micron sections dry with a glass knife; or thinner
sections with a glass knife equipped with a boat filled with distilled
2) use a forceps for the dry-cut sections or a tapered wooden pick for
the wet-cut sections to place them on a dome of distilled water on the
Superfrost Plus slide
3) heat on a hot plate that is set a little less hot that that which
would make the water on the slide boil (too hot to touch). Place an
old glass knife on the hot plate so that the slide can be propped up
on it at the frosted (handling) end.
4) let the water under the section evaporate and then wait 20 seconds
before removing the slide from the hot plate and staining
5) stain with filtered toluidine blue, by placing drops of stain so
they dome over the sections and placing the slide flat on the hot plate
for 15-20 seconds. Don't let the stain boil or dry out.
6) tap off the stain and quick rinse in 3 changes of distilled water,
one change in 70% ethanol and then one more change of distilled water
and place the section back on the hot plate at an angle, over the old
glass knife until dry
We have had very good luck with this protocol, in fact we have none of
the sections releasing even if we have to remove the coverslip and
Karen Pawlowski, Ph.D.
University of Texas at Dallas
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