RE: attaching MMA thin sections to slides

From:Fred Underwood

Hi Sarah,
For paraffin sections of rib or ear I use a product named Titebond 2, about a 2% aqueous solution.  I found it at Lowe's but is probably available at any hardware store.  For me it works better that gelatin, elmers glue, or even charged slides.
Fred Underwood

	-----Original Message-----
From: Sarah Jones  
Sent: Monday, February 10, 2003 5:11 PM
Subject: attaching MMA thin sections to slides

	Hello all you Uncle Ben fans, hope those that need to concentrate on 
their breathing are doing well, 
  I would appreciate any procedure on MMA thin sections in regards to 
attaching them to the slides.  Specifically, the steps including 
sectioning through deplastinization.  I've been having trouble keeping 
them on the slides with Subbed Slides and Plus Coated.  How are the 
sections floated out?  
PS  I like the suggestion about using OT, thanks for the reminder.  I 
think it is better to use the delete button than to get your blood 
pressure up.  We don't need to lose any precious histotechs to heart 
attacks.  I'm sure we would all like to become like the retired guy for 
a few years before that happens.  Personally, I like a few off topic 
subjects and OT would help those that don't.  

	Sarah Jones HT(ASCP) 
Dept. of Vet. Anatomy & Public Health 
Histology Lab 
Texas A&M University 
College Station, TX 77843 4458 
phone: 979 845 3177 
fax:  979 458 3499 

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