RE: PDGFR

From:

jim

Lil

I can only supply a protocol for PDGFRalpha. We use a polyclonal antibody from Santa Cruz (sc-338). Retrieval on paraffin blocks is done using DAKO's TRS solution at a dilution of 1/400 with overnight incubation.

Cheers

Jim Manavis
Senior Hospital Scientist
Department of Neuropathology
Institute of Medical & Veterinary Science
Adelaide, SA, 5000
Australia
Phone: 61-08-8222-3668
FAX: 61-08-8222 3204
e.mail: jim.manavis@imvs.sa.gov.au
Disclaimer: Not this little black duck!

-----Original Message-----
From: Lilia Muolo [mailto:muololi@umdnj.edu]
Sent: Thursday, 6 February 2003 04:28 AM
To: Histonet@pathology.swmed.edu
Subject: PDGFR

Hi,

Does anyone have good protocols for PDGFR (a & b) immuno. staining on paraffin blocks. And please indicate which company has good antibodies as the ones I tried are not working. Thanks in advance.

Lil Muolo

Immunohistochemical lab

Cancer Institute of New Jersey

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