The
tissue needs to be fresh, snap frozen in liquid nitrogen. About a 1-2cm piece
should be sufficient. Part should be minced and put in glutaraldehyde for
possible Em. The frozen tissue is cut on a cross section. There are many
immuno antibodies for dystrophys, sarcoglycans, distrophyins etc., along with
routine frozen muscle stains including gomori, nadh, atpase. Good Luck, rwrite
back if you need more info.
Annette Featherstone HT/ Kaleida Health
I heard that
muscular dystrophy patients can further type the type of MD they have by
getting tests done on a muscle biopsy. Does anyone know the most
recent testing done on these biopsies? How big the tissue needs to be?
If anyone has info on this matter or can further direct me, please let
me know.
Thanks,
Christina DeBari
Cytology Supervisor
Holy Name
Hospital
Teaneck, NJ ----
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