RE: Meconium in placental membranes

From:Tony Henwood

RE: Meconium in placental membranes


AND MUCONIUM IN FOETAL LUNG". A method was posted by Ron Rainbow on Histonet in May last year (I think).

Reference:Attwood H.D.:The Histological Diagnosis of Amniotic Fluid Embolism. Journal of Pathology and Bacteriology. Vol.76; pg211; 1958.

Since alcian green is rare, I wonder if one could use alcian blue instead?

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: Steve Machin UK []
Sent: Tuesday, 4 February 2003 21:05
To: Histonet Histonet Histonet
Subject: Meconium in placental membranes

Has anyone been able to get a positive staining reaction for meconium
in placental membranes?
We have tried the usual bile stains and they are negative, Fouchet,
Stein and Gmelin.
My understanding is the the pigment in meconium is bilirubin, the
meconium, seen in macrophages, comes up negative but our controls are

Best Wishes
Steve machin UK

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