Elizabeth,
I do Connexin 43, which is a gap junction protein antibody, on FFPE tissue.
If this is what you are looking for give me a shout and I will forward the
protocol on to you.

Mary Georger
Center for Cardiovascular Research
University of Rochester Medical Center
KMRB Room 2-9816
601 Elmwood Avenue.
PO Box 679
Rochester, New York  14642
585-273-1548

> ----------
> From: 	Elizabeth Chlipala
> Sent: 	Thursday, February 6, 2003 11:59 AM
> To: 	HistoNet Server
> Subject: 	GAP 43
> 
> Has anyone out there done immunostaining for GAP 43 on 40 micron vibratome
> brain sections?  Also I have never worked with vibratome sections or done
> work on free floating sections.  Is there a reference or protocol with a
> list of supplies, so I can see what I'm getting into and what I would need
> to purchase.
> 
> Thanks in advance
> 
> Liz Chlipala
> BolderPATH
> 
> 
>