Taren,
I have a protocol and recipes, unfortunately not in my data base, but if you
send me your fax # I will send you a good old fashion hard copy! Out of
curiosity, what smooth muscle marker are you using? We are  smooth muscle
group:)
Mary Georger
Center for Cardiovascular Research
University of Rochester Medical Center
KMRB Room 2-9816
601 Elmwood Avenue.
PO Box 679
Rochester, New York  14642
585-273-1548

> ----------
> From: 	David Walker
> Sent: 	Monday, February 3, 2003 2:44 PM
> To: 	Histonet
> Subject: 	Borax treatment
> 
> I am posting this for a Graduate Student:
> Dear Histonetters-
> I am doing a double-labeling immunohistochemistry protocol for smooth
> muscle
> and BrdU.  The method calls for an HCl treatment to denature the DNA
> followed by a Borax treatment to stabilize the DNA.  Does anyone have a
> Borax treatment protocol, including recipes?  Thank you in advance for
> your
> responses.
> 
> Regards,
> Taren Grass
> 
> Dave
> 
> 
>