-----Original Message-----
From: Nymeyer, Heather [mailto:Heather.Nymeyer@interiorhealth.ca]
Sent: Thursday, February 13, 2003 19:49
To: 'histonet@pathology.swmed.edu'
Subject: Bielchowsky stain

I need some help in reducing or eliminating the amount of background silver precipitate on the slide when performing a Bielchowsky stain. The method being used is a 20 % silver nitrate solution for 20 mins followed by a 15 min incubation (in the dark) of ammoniacal silver.  The reducer consisting of citric acid, formaldehyde, water.  A few drops of the reducer is added to the ammoniacal silver and checked microscopically until fibers are seen.  At the same time the fibers are being developed the excess background precipitate is forming.    Any suggestions or solutions would be greatly appreciated. 

----
CAUTION
This email message was received from an email system outside of Kaleida Health. Use caution when opening email attachments as they are a major source of virus infections. Do not open attachments that appear not to be for Kaleida business purposes, such as games, jokes, and screen savers. Computer viruses can send email messages that appear to be from people that you know and you could end up sending them on to everyone in your own address list.
----