IHC on spinal cord sections
We are several persons in the lab who perform immunohistochemical
revelation on floating spinal cord sections after PAF4% transcardiac
perfusion (with or without picric acid). It often appears a staining of
blood vessels which seems to be non specific. It's hard to explain because
it does not appear on sections incubated without the first antibody -so it
should be specific- but the staining is smooth and fills the entire cavity
of the blood vessels. Moreover it often does not concern the entire section
and is regionalized. We have this problem either with fluo or with
perox-revelation (but it is more frequent with the latter).
I've tried to change my protocol (with or without serum in the buffer), but
it doesn't change anything...
I've made IHC on brain and medulla sections of the same animals and this
problem appeared very rarely .
Does someone have an idea ?
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