IHC for Acetylcholinesterase?
Agreed . One could use tinctorial methods in confirming abscence or even
reduced numbers of Ache +ve ganglia/fibres but as you state....difficult
.Immuno against Ache itself or NCAM or NF for eg will unequivocally
demonstrate these cells/processes. However, I put this Q to the great Brian
Lake at Grt Ormond St Childrens Hospital UK a few years ago and he replied ,
if it aint broke, don't fix it! ( using IHC as an alternative to detection
of enzyme activity)
I have used IHC in Pwax trials and it looked good...( thinking of the hassle
of frozen sectioning)but I'm not an expert. Then I left clinical labs.
Molecular Neurobiology Group
MRC Centre For Developmental Neurobiology
New Hunts House
kings College London
020 7848 6810/fax 6816
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